| Development of Hyaluronic Acid-Based Skin Patch Containing Nanocellulose for Wound Healing Applications |
| Paper ID : 1298-IGA |
| Authors |
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Mahsa Ahmadi Fasih * Neda-ye Zahra private school, Tehran, IRAN |
| Abstract |
| Background and Aim: Effective wound management requires dressings that accelerate tissue repair, maintain moisture, and prevent infection. Conventional dressings such as gauze and cotton often fail to provide adequate healing conditions. This study aimed to design and evaluate a novel skin patch composed of bacterial nanocellulose incorporated with hyaluronic acid (HA) for improved wound healing outcomes. Materials and Methods: Bacterial nanocellulose was synthesized using Stobachther xylinium cultures, purified, and combined with hyaluronic acid. The patch was characterized through structural and cytotoxicity assays. Cell viability was assessed using NIH3T3 fibroblasts via MTT assay. Antibacterial activity was evaluated against pathogenic bacterial strains. An in vivo wound healing model was conducted on male rats, comparing treated and control groups over a 7-day period. Results: The MTT assay confirmed biocompatibility of the patch, with fibroblast cell viability above 90% at concentrations ≤0.1 mg/ml. Antibacterial tests revealed an inhibition zone of approximately 14 mm for the nanocellulose–HA patch compared to 11 mm in the control. In vivo evaluation showed a significant reduction in wound diameter: from 1.5 cm at baseline to 0.4 cm by day 7 in the treated group, versus 1.0 cm in controls. Conclusion: The developed nanocellulose–hyaluronic acid skin patch demonstrated excellent biocompatibility, antibacterial activity, and enhanced wound healing in vivo. These findings suggest its strong potential as an advanced wound dressing for clinical applications. |
| Keywords |
| Wound healing, Skin patch, Bacterial nanocellulose, Hyaluronic acid, Antibacterial activity |
| Status: Accepted |
