| The Inhibitory Effects of Green Synthesized Selenium Nanoparticles on Migration of Ovarian Cancer (OVCAR-3) Cells |
| Paper ID : 1293-IGA |
| Authors |
|
Fatemeh Siadat * Tehran - Faculty member of the Islamic Azad University, North Tehran Branch. |
| Abstract |
| Background and Aim: Ovarian cancer remains a leading cause of cancer-related mortality among women, with metastasis driven largely by cancer cell migration and proliferation. Selenium nanoparticles (SeNPs), especially those synthesized via green methods, have shown promise as potential anti-cancer agents. This study aimed to evaluate the effects of SeNPs, green synthesized using Onopordum acanthium extract, on the migration and proliferation of OVCAR-3 ovarian cancer cells. Methods: SeNPs were biosynthesized using Onopordum acanthium extract and applied to cultured OVCAR-3 cells. A scratch (wound healing) assay was performed to assess cell migration and proliferation over 72 hours in both treated and untreated control groups. Images were captured at 0 and 72 hours to qualitatively evaluate wound closure. Results: In the control group, OVCAR-3 cells showed extensive migration and/or proliferation, resulting in near-complete closure of the scratch area after 72 hours. In contrast, the SeNP-treated cells exhibited significantly reduced wound closure, with the scratch area remaining visibly open and only partially filled by migrating/proliferating cells. These observations suggest that SeNPs significantly inhibit the migratory and proliferative capabilities of OVCAR-3 cells. Conclusion: Green-synthesized selenium nanoparticles using Onopordum acanthium extract effectively suppress cell migration and proliferation in OVCAR-3 ovarian cancer cells in vitro. This indicates their potential as anti-metastatic agents in ovarian cancer therapeutics. Further quantitative and mechanistic studies are warranted to validate these findings. |
| Keywords |
| Selenium nanoparticles, Green synthesis, Onopordum acanthium, OVCAR-3, Cell migration inhibition |
| Status: Accepted |
