| Spent Coffee Grounds as a support for Sustainable Biofertilizers |
| Paper ID : 1278-IGA |
| Authors |
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Sameh Ben Mabrouk *, Bouthaina Ben Hadj Hmidaa, Wejdene Sellami, Ahmed Aloulou Laboratory of Biochemistry and Enzyme Engineering of Lipases, ENIS, Sfax, Tunisia |
| Abstract |
| Background and Aims: Coffee production and processing generate significant volumes of waste, particularly spent coffee grounds (SCG). Their recovery is part of a circular bioeconomy approach. This work explores the use of SCG as a matrix for the immobilization of a bacterial amylase for use in fertilization. Material and Methods: Amylase from Bacillus strain sp and spent coffee grounds (SPG) were used to prepare the fertilizer agent. Three different plant species (broad bean : Vicia faba, lentil: Lens culinaris, chickpea : Cicer arietinum) were used for the tracking of the root growth of plants basing on the number of survival stems, the number of leaves per stem, and the avearage length of stems. Results: The immobilization of α-amylase from Bacillus sp on spent coffee grounds was evolved. After the optimization process, the immobilization efficiency was increased 5 times thanks to the optimized condition including an immobilization pH and temperature of ≈ 7.5 and 4°C, reaction time of 10 minutes, contact time of 45 minutes, and agitation speeds of 50 rpm. The immobilized enzyme exhibited a significantly greater fertilizing effect than coffee grounds used on their own. Indeed, depending on the species tested, the growth rate was increased by a factor ranging from 1.5 to 2.5. In addition, the immobilized enzyme's storage capacity was tried to compare to the free enzyme. After 25 days of storage at 25°C, the immobilized enzyme retained 70% of its activity, whereas the free enzyme dropped its activity inside of 3 days., Conclusion: This study highlights the potential of SCG as an effective and sustainable matrix for enzyme immobilization, offering a dual benefit of waste valorisation and enhanced agricultural productivity. |
| Keywords |
| Spent coffee grounds; adsorption; amylase;; Biofertilizer |
| Status: Accepted |
