| Crosslinking gelatin with robust inherent antibacterial natural polymer for wound dressing application |
| Paper ID : 1267-IGA |
| Authors |
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Raziyeh Najafloo *1, Noshad Peyravian2, Zahra Pezeshkian2, Peiman Brouki Milan2, Afzal Karimi2 1Department of biomedical engineering, TeMS.C., Islamic Azad University, Tehran, Iran 2Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran 1449614535, Iran |
| Abstract |
| Background and Aim: Gelatin-based biomaterials are widely acknowledged as a promising choice for wound dressings, given their similarity to the extracellular matrix and biocompatibility. However, the challenge of cross-linking gelatin while preserving its biocompatibility and cost-effectiveness persists. This study aimed to enhance the properties of gelatin by incorporating the oxidized lignosulfonate (OLS) biopolymer as an inexpensive and biocompatible natural material. The polyphenolic structure of OLS acts as both a cross-linking agent and an antibacterial component. Materials and Methods: The OLS/gelatin films were prepared using a casting method with varying weight ratios (0.1, 0.2, 0.3, 0.4, and 0.5 w/w). The physicochemical features of fabricated films were analyzed by FTIR, swelling and degradation rate as well as mechanical strength. Also, the safety and efficacy were evaluated following an antibacterial assay, in vitro study, and wound healing animal model. Results: FTIR analysis confirmed the formation of Schiff-base and hydrogen bonds between gelatin and OLS. The resulting films exhibited enhanced mechanical properties (Young's modulus ~40 MPa), no cytotoxicity, and excellent cell adhesion and morphology. Antimicrobial tests showed significant activity against Escherichia coli and Staphylococcus aureus, with higher activity against S. aureus (17 mm inhibition zone and 99% bactericidal rate). In vivo studies in a mouse model demonstrated that the gelatin/0.2OLS dressing significantly improved wound healing, including re-epithelialization, collagen formation, inflammation reduction, and blood vessel density, compared to untreated wounds Conclusion: These findings suggest that the synthesized novel gelatin/OLS wound dressing has promising healing and antibacterial properties. |
| Keywords |
| Gelatin, Oxidase lignosulfonate, Wound healing, Film, Cross-linking |
| Status: Accepted |
