| Integrating gene Therapy and Immunotherapy in Precision Medicine: Novel Approaches in Targeted Cancer Treatment |
| Paper ID : 1240-IGA |
| Authors |
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Hesam Aminian *, Parinaz Khanjanpoor Department of Health and Science, School of Medicine, University of Piedmont Orientale (UPO), Novara, Italy |
| Abstract |
| Background and Aim: Integrating gene therapy and immunotherapy represents a cutting-edge approach in precision medicine. This review focuses on novel strategies combining gene-editing technologies, such as CRISPR-Cas9, with immunotherapeutic modalities like CAR-T cells and immune checkpoint inhibitors to enhance efficacy and personalize cancer care. Materials and Methods: A systematic literature search was conducted using keywords including "gene therapy," "immunotherapy," "precision medicine," "cancer," "CAR-T," and "CRISPR" across databases such as PubMed and Frontiers in Medicine. Inclusion criteria were peer-reviewed articles published in English from 2015 to 2025, excluding conference proceedings. Results: Gene therapy advances enable precise targeting of cancer-driving mutations and enhancement of antitumor immune responses. Combining gene therapy with immune checkpoint inhibitors improves T-cell persistence and overcomes tumor immunosuppression, particularly in solid tumors. CRISPR-edited CAR-T cells with multi-antigen targeting address tumor heterogeneity and antigen escape, showing promising preclinical and early clinical results. Additionally, combination approaches integrating gene therapy, chemotherapy, radiation, and ferroptosis inducers synergistically enhance tumor cell killing and immune activation. Emerging delivery systems, such as lipid nanoparticles and exosomes, improve safety and targeting efficiency. Parallel advances in patient-derived organoids combined with CRISPR screening provide powerful platforms to identify therapeutic targets and optimize personalized immunotherapies. Conclusion: The integration of gene therapy and immunotherapy in precision oncology offers transformative potential for targeted cancer treatment. Combination strategies leveraging gene editing, CAR-T cells, and immune modulation overcome key challenges such as immune evasion and tumor heterogeneity. |
| Keywords |
| Gene therapy, Immunotherapy, Precision medicine, Cancer |
| Status: Accepted |
