| Differential Viability of Ovarian Cancer and Normal Cells Exposed to Green SeNPs |
| Paper ID : 1235-IGA |
| Authors |
|
Ali Mehdiaraghi *, Fatemeh Siadat Department of Biology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran. |
| Abstract |
| Background and Aim: Green synthesis of selenium nanoparticles (SeNPs) offers a biocompatible strategy for cancer therapy. This study evaluates the cytotoxicity of green-synthesized SeNPs on ovarian cancer cells (OVCAR-3) compared to normal human embryonic kidney (HEK) cells, aiming to determine their selectivity and potential therapeutic index. Materials and Methods: SeNPs were synthesized using an eco-friendly method and applied to OVCAR-3 and HEK cell lines at concentrations ranging from 15.625 to 500 µg/ml. Cell viability was measured using the MTT assay after 24 hours. Data were analyzed to determine dose-response relationships and relative sensitivity of the cell types. Results: SeNPs reduced viability in both cell types in a concentration-dependent manner. In OVCAR-3 cells, viability decreased sharply from 97.73% at 15.625 µg/ml to 11.63% at 500 µg/ml. HEK cells also showed reduced viability at high concentrations (12.19% at 500 µg/ml), but maintained over 77% viability at concentrations up to 125 µg/ml. Notably, OVCAR-3 cells were more sensitive at mid-range doses (e.g., 57.06% vs. 77.41% at 125 µg/ml), suggesting selective cytotoxicity. Conclusion: Green-synthesized SeNPs exhibit potent, dose-dependent cytotoxic effects on ovarian cancer cells, with relatively lower toxicity toward normal HEK cells at moderate concentrations. This selective effect underscores the potential of SeNPs as a targeted agent in ovarian cancer treatment, meriting further mechanistic and in vivo exploration. |
| Keywords |
| Selenium nanoparticles, Green synthesis, OVCAR-3, HEK cells, Cell viability |
| Status: Accepted |
