| Assessment of Oxidative Potential in Culture Medium Conditioned by MCF7 Cancer Cells via FRAP and MDA Assay |
| Paper ID : 1193-IGA |
| Authors |
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Ahmadreza Gholamian * Department of Science, Faculty of Veterinary Medicine, Ferdowsi University, Mashhad, Iran |
| Abstract |
| Background and Aim: In recent years, the use of natural-origin drugs has gained significant attention due to their lower harmful effects compared to conventional chemical drugs. Recently, fungi and yeasts have been studied as rich sources of effective biological compounds. This research aims to investigate the oxidative effects of the cytoplasmic extract of Saccharomyces cerevisiae on the MCF-7 cancer cell line. The objective of this study is to evaluate the antioxidant potential of this extract and analyze the changes in oxidative markers in these cells. Materials and Methods: Yeast cells of Saccharomyces cerevisiae (PTCC 5052) were cultured, harvested, disrupted using sonication, and cytoplasmic fractions were extracted via ultracentrifugation. MCF7 breast cancer cells were cultured and treated with yeast cytoplasmic extract. The antioxidant potential (FRAP) and oxidative marker (MDA) were measured at different concentrations and time points using spectrophotometry. Statistical analyses were performed with SPSS version 28. Results: The results of the FRAP and MDA tests indicate that, except for cases where the cells were treated for 72 hours, no significant differences were observed in other cases. Additionally, the normal distribution of the data can be observed using the qq.plot chart. Conclusion: Additionally, in examining the antioxidant marker FRAP and the oxidative marker MDA, it was observed that there were no significant differences in the FRAP test between the control samples and the tested samples. Except for the 48 and 72-hour time points at a concentration of 2000 µg/ml, there was no considerable decrease compared to the control sample, making it impossible to conclusively determine that the reduction in this antioxidant marker caused cell death. Similarly, in the evaluation of the oxidative marker MDA, similar results were concluded. |
| Keywords |
| Saccharomyces cerevisiae, Breast cancer, Antioxidant activity |
| Status: Accepted |
