| Unveiling the Hidden Universe: Microbial Biodiversity Profiling of Rice Paddy through a Dual Lens - A Culture-Dependent and Independent Approach |
| Paper ID : 1117-IGA |
| Authors |
|
Ibrar Khan * Department of Microbiology,
Abbottabad University of Science
& Technology, Abbottabad Pakistan. |
| Abstract |
| Rice (Oryza sativa), a global staple and nutritional powerhouse, thrives in a delicate dance with its underground partners, the rhizobiome. This intricate web of communication between plant and bacterial communities holds the key to the plant's growth and productivity, ultimately shaping the fate of the crop. In this groundbreaking study, we delve into the hidden world beneath the rice fields, employing both culture-dependent and culture-independent techniques to unravel the bacterial diversity surrounding rice roots. The microbial composition of rice paddy soil exhibited a distinctive profile, with Proteobacteria representing the least common phyla, displaying a relative abundance ranging from 0.00 to 0.50%. At the genera level, Prevotella, Rheinheimera, and Rhizobium emerged as the most abundant genera, with relative abundances ranging from 0.75 to 1.00%. Notably, our investigation revealed that bacterial community structure was predominantly influenced by the relative abundance of bacteria rather than the specific species. Among the identified bacteria, Pseudomonas (Proteobacteria), Synergistetes, Actinobacteria, Geobacter (Proteobacteria), Firmicutes, Bacteroidetes, and Cyanobacteria were found to be the most prevalent in descending order. Employing a screening approach of soil-based libraries with functional and sequence-based criteria, our study unveiled novel microbial communities, offering valuable insights into the intricacies of soil microbial ecology. The soil microbiome, acting through biocontrol mechanisms, plays a crucial role in supporting plant growth. Furthermore, our investigation concluded that these bacterial communities exert a significant influence on key soil processes, including carbon sequestration, nitrogen cycling, and soil fertility maintenance. The rhizobiome's impact on the plant was observed to be both direct and indirect. Importantly, our study elucidated the modulation of the rhizobiome throughout different stages of rice development, providing a foundation for the targeted application of bio-fertilizers tailored to each cultivation stage. This nuanced understanding enhances the potential for optimizing agricultural practices and fostering sustainable cultivation methodologies. |
| Keywords |
| Rice (Oryza sativa), Rice Paddy, Rhizobiome, Metagenomic Analysis |
| Status: Accepted |
